B-Glucuronidase

Dray J, Dray F, Tiller F, Ulman A (1972) Hydrolysis of urine metabolites of different steroid hormons by b-glucuronidase of Escherichia coli. Ann Inst Pasteur 123 853-857... 

Careful examination of the yellowish sediment obtained after spinning down the crude mitochondrial fraction showed it was frequently overlaid with loosely packed, fluffy material —the fluffy layer. Experiments from de Duve s and, later, Novikoff s laboratories in the 1950s demonstrated that the lighter, lysosomal fraction was enriched in a number of hydrolases including acid phosphatase, aryl sulphatase, B glucuronidase, RNAase, and a peptidase, cathepsin. All the enzymes had optimal pHs in the acid range (pH 5-pH 6). Density... 

The following procedure was developed for urinary pregnanediol, pregnanetriol, and 17-ketosteroids in urine. It is similar to the one presented earlier for pregnanediol (12). Hydrolysis is complete because sulfatase for the cleavage of sulfates is included with the B-glucuronidase. 

Jefferson, R.A., Kavanaugh, T.A. and Bevan, M.W. (1987) GUS fusions B-glucuronidase asa sensitive and versatile gene fusion marker in higher plants, EMBO J. 6, 3901-3907... 

Csala M, Banhegyi G, Braun L, Szirmai R, BurcheU A, Burchell B, Benedetti A, Mandl J. B-glucuronidase latency in isolated murine hematocytes. Biochem Pharmacol, 2000 59(7) 801 05. 

The other two metabolites identified (Mi and Mg) are glucuronic acid conjugates since hydrolysis by B-glucuronidase produces iopronic acid (M3) and 2-hydroxylethyl(3-acetamido-2,4,6-triiodo-phenyl)ether (Mi ) respectively. The structure of Mg was confirmed by TLC, HPLC and by comparison with an authentic sample prepared as shown in Fig. 10. The mass spectrum of Mg is shown in Fig. 11. 

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