B Determining GLC Response Factors

Purpose To determine the weight and mole correction factors for compounds using flame ionization or thermal conductivity detectors. 

The solvents and other liquids that are used in sample mixtures for GLC analysis are flammable and volatile. No flames should be used in the vicinity of these liquids. 

Preparation Sign in at www.cengage.com/login to answer Pre-Lab Exercises, access videos, and read the MSDSs for the chemicals used or produced in this procedure. Read or review Section 2.5. 

Setting Up Since there is considerable variation in the operating procedures of different commercial gas chromatographs, consult your instructor for specific directions to use the instrument in your laboratory. Look up the densities of the compound 

Injection Following the directions of your instructor, use a gas-tight syringe to inject a 1-5-jjlL sample of the test mixture into the GLC to obtain a chromatogram. Repeat this process for each of the compounds for which you are to obtain weight and mole response factors. 

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