Axenically grown

Mobilization of inicronutrients such as Zn, Mn, Cu, and Co and of heavy metals (Cd, Ni) in soil extraction experiments with root exudates isolated from various axenically grown plants is well documented (61,204-206) and has been related to the presence of complexing agents. 

Y. Wang, H. N. Brown, D. E. Crowley, and P. J. Szaniszlo, Evidence for direct utilization of a siderophore, ferrioxamine B in axenically grown cucumber Plant Cell Environ. 76 579 (1993). 

Booden, T., Albach, R. A. and Boonlayangoor, P. (1978) Uptake of selected pyrimidines by axenically grown Entamoeba histolytica. Arch. Invest. Med. 9 Suppl. l(Mex) 133-140. 

Exudates of various kinds isolated from axenically grown plants have been shown to complex metals (Morel et al, 1986 Gries et al, 1995). Hamon et al... 

Leaf and root explants from axenically grown seedlings of L fendleri regenerate shoots profusely on MS medium with 3% sucrose and 1 mg/L each of benzyl adenine and zeatin. These explants were cocultivated Agrobacterium tumefaciens harbouring a binary transformation vector whose T-DNA carries genes conferring resistance to either the herbicide L-phosphinothricin (L-PPT) or to kanamycin. 

The Cyanophyta (Cyanobacteria) were once thought to contain no sterols at all (1). The most significant characteristic about sterols in Cyanophyta is that they are usually present in much smaller amounts than in other photosynthetic plants. Many species are difficult to obtain in pure culture and analysis of non-axenically grown blue-greens is risky due to the ease of... 

However, axenlc cultures were grown in ESNW only, and only those extracts which supported the best growth in bacterlzed cultures were used in the axenic culture experiments. 

The haustoria of the parasites do not form when the plants are grown axenically, but are rapidly induced in the presence of the host roots or host root exudates (17). Several haustorial-inducing compounds have now been characterized. Xenognosin A and B were identified in gum tragacanth, an exudate of Astragalus gummifer. and soyasapogenol B was identified in roots of Lespedeza sericea. 

Axenic cultures of Agalinis grown in the absence of a host develop few or no haustoria (4,11). Induction of this organ is rapid upon... 

Clones of duckweed plants were grown axenically in Stewart s growth medium (17) with asparagine at 132.1 mg/L as nitrogen source. Cultures were maintained in 250-mL Erlenmeyer flasks with 100 mL of medium per flask in controlled environment rooms... 

Fig. 5 Daily specific growth rates of total DMS + DMSPd + DMSPp versus specific cell growth in a variety of axenic Phaeocystis globosa batch cultures under different conditions. Cultures were grown at the same temperature (11°C), but with different salinities, nutrient ratios and/or light conditions. Specific growth rates were calculated per day. Culture growth details are as follows N-sal salinity range from 25 to 50 PSU, nitrogen limited P-sal salinity range from...

The plant S. japonica (2 years old), grown in the field, was collected and used for the establishment of the shoot cultures. The deleaved fresh shoots, after surface sterilization with 2 % NaClO with Tween 20 (see 2.2.1), were cultured on hormone-free 1/2 MS medium in the light (16 hr photoperiod/day). A. rhizogenes 15834 strain harbouring Ri plasmid (pRi 15834) was inoculated by a needle onto the cut ends of the axenic stems. Two to 4 weeks after inoculation, hairy roots appeared at the inoculated sites. The hairy roots, after removal of the bacteria, were transferred and maintained on hormone-free RC solid medium in the dark. 

Eehrmann, C., Pohl, P, 1993. Cadmium adsorption by the non-living biomass of microalgae grown in axenic mass culture. Journal of Applied Phycology, 5, 555-562. 

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