Auxins solvents

Solvents. The acid and ammoniacal solvents (Table 89, XIV and XVI), suggested by Stahl and Kaldewey [120], have been in the meantime (with some modifications — Table 89, XV and XVII) applied successfully to separation of auxins and urine metabolites. 

Caution is demanded when ammoniacal or acid solvents are used in investigations on plant auxin metabolism. Indole-3-pyruvic acid and glucobrassicin decompose during TLC in ammonia-containing media. Good separation of a number of indole derivatives is possible in solvents... 

The following solvent combinations, in order of use, have been so far proposed (Roman numerals refer to Tables 88/89, capital letters to Table 90) XIV -> XVI a [120] and V->IV [19] for separating simple indole derivatives, particularly from auxin metabolism (the second combination is suitable for the methyl esters of the acids also) XVII-> XIV [12], XV-> XVIa [9] isopropanol-25% ammonium hydroxide-water (80 + 4 + 8) IX [90] for separation of urine metabolites A B for hydroxyskatoles and C D for hydroxyindoles. 

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