Autosampler-operating Guides

Use a methanol or acetonitrile and water mixture without buffer (e.g., 50%) as the flush solvent for most autosamplers. Degas it to avoid bubbles. 

Fill the vial with enough sample solution for all injections. Note that some antosamplers nse a side-port needle that might reqnire at least a 0.5 mL sample volnme in a 2-mL vial (e.g., Waters Alliance). 

Purge the injector daily and before sample analysis to remove bubbles in the sampling syringe. This is critical for sampling precision. 

Typical injection volnmes for pharmacentical assays range 5-50 pL. Injection sizes of 5pL might lead to poorer precision. 

Put a warning label ontside the antosampler if a non-standard sampling syringe or loop is installed. 

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