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Automated method screening columns/mobile phases

Step 6 Screen Different Columns/Mobile Phases Using an Automated Method Development System or Column Switcher. If the desired selectivity is not obtained or the peak is not spectrally homogeneous from Step 5, then different columns (two to six) and mobile phases can be screened using a column switcher. The typical mobile phases that could be screened include acetonitrile, methanol, and acetonitrile/methanol mixtures using the same "pH... [Pg.417]

To conduct the method screening process, each mobile phase is run on each column, excluding combinations that are incompatible. This can be performed in automated fashion with modern HPLC systems using a column-switching valve and running the separate candidate mobile phases sequentially on each column. To set the additional HPLC variables, it is recommended that the column set at 35-40 °C to reduce mobile-phase viscosity and improve separation efficiency (N) versus ambient conditions. [Pg.354]

See other pages where Automated method screening columns/mobile phases is mentioned: [Pg.295]    [Pg.152]    [Pg.413]    [Pg.416]    [Pg.417]    [Pg.155]    [Pg.7]    [Pg.8]    [Pg.291]    [Pg.222]    [Pg.429]    [Pg.54]    [Pg.511]    [Pg.411]    [Pg.601]    [Pg.6]    [Pg.6]    [Pg.406]    [Pg.176]   


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Automated methods

Automated screening

Automation screening methods

Columns method

Method phase

Method screening

Mobile phase column

Mobile phase screening

Mobility methods

Screening automation

Screening-Methode

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