Atomic absorption spectrometry coupled with HPLC

Figure 6.1 Bar-graph of MeHg in CRM 580. The results correspond to six replicate determinations as performed by different laboratories using various methods. MEANS indicates the mean of laboratory means with 95% confidence interval. Abbreviations-. CVAAS, cold vapour atomic absorption spectrometry CVAFS, cold vapour atomic fluorescence spectrometry ECD, electron capture detection GC, gas chromatography HPLC, high-performance liquid chromatography ICPMS, inductively coupled plasma mass spectrometry MIP, microwave induced plasma atomic emission spectrometry QFAAS, quartz furnace atomic absorption spectrometry SFE, supercritical fluid extraction.

In addition, for speciation coupling of flow injection analysis and column chromatography with flame AAS and also a direct coupling of HPLC with flame AAS, as is possible with high-pressure nebulization, are most powerful. Here the Cr line in the visible region can be used, which makes the application of diode laser atomic absorption spectrometry possible [325]. This has been shown recently by the example of the determination of methylcyclopentadienyl manganese tricarbonyl. 

Le, X.-C., Cullen, W. R., and Reimer, K. J. (1994). Speciation of arsenic compounds by HPLC with hydride generation atomic absorption spectrometry and inductively coupled plasma mass spectrometry detection. Talanta 41(4), 495. 

A very recent volume edited by Berthed (2002) is on countercurrent chromatography - the support-free liquid stationary phase. Ebdon et al. (1987) review directly coupled liquid chromatogramphy-atomic spectroscopy. The review by Uden (1995) on element-specific chromatographic detection by atomic absorption, plasma atomic emission and plasma mass spectrometry covers the principles and applications of contemporary methods of element selective chromatographic detection utilizing AA, AES and MS. Flame and furnace are considered for GC and HPLC, while MIP emission is considered for GC and ICPAES for HPLC. Combinations of GC and HPLC with both MIPAES and ICPAES are covered and supercritical fluid chromatographic (SFC) and field flow fractionation (FFF) are also considered. 

Note AAS Atomic absorption spectroscopy ICP-MS Inductively coupled plasma-mass spectrometry MAFF Ministry of agriculture fisheries and food HPLC-UV High performance liquid chromatography with UV detection ASV Anodic stripping voltammetry CSV Cathodic stripping voltammetry. 

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