Assay singlet oxygen

Keywords histidine assay, singlet oxygen, anti-bacterial, photochemical activity, quinolone, reactive oxygen species... 

Di Mascio P., Sandquist A., Devasagayam T., and Sies H. (1992). Assay of lycopene and other carotenoids as singlet oxygen quenchers. Methods Enzymol 213 429 438. 

The palladium(II) phthalocyanines are satisfactory singlet oxygen generators. Thus, f>A values for tetra-t-butylphthalocyanine ((27), with stated replacement for Mg) are 2H, 0.22 Zn, 0.34 Pd, 0.54, i.e., increasing in line with the heavy atom effect.180 For palladium(II) tetra-t-butylphthalocyanine ((27), Pd instead of Mg) in benzene at 290 K, 4>f= 0.048 and X = 0.49.180 However, palladium(II) 2,3-dihydroxy-9,16,23-tri-t-butylphthalocyanine was synthesized by a mixed condensation (1 9 mix of appropriate dinitriles) as a likely amphiphilic sensitizer, but did not show bioactivity in an enzyme assay, possibly because of aggregation.180... 

Vileno B, Lekka M, Sienkiewicz A, Marcoux P, Kulik AJ, Kasas S, Catsicas S, Graczyk A, Forro L (2005) Singlet oxygen ((l)Delta(g))-mediated oxidation of cellular and subcellular components ESR and AFM assays. Journal of Physics-Condensed Matter 17 S1471—S1482. 

AlphaScreen (amplified luminescent proximity homogeneous assay) PerkinElmer Life Sciences Donor beads produce singlet oxygen that induces fluorescence in proximal acceptor beads bead-bead proximity results from engagement of binding partners immobilized on the two bead types... 

Table 13. Singlet oxygen decontaminase activity 0/ Cu-chelales and erythrocuprein. The erythrocuprein concentration required to produce a 50% inhibition of the chemiluminescence was taken an enzyme unit for quantitating the singlet oxygen decontaminase activity. The assay mixture contained HEPES buffer, 0.1 M, pH 7.8 luminol, 0.3 mM crystalline K3CrO%, 0.3 mM the total volume was 5.1 ml. Counting was performed as described in the legend to Fig. 32...

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