Assay peroxyoxalate

Applications of the oxalate-hydrogen peroxide chemiluminescence-based and fluorescence-based assays with NDA/CN derivatives to the analysis of amino acids and peptides are included. The sensitivity of the chemiluminescence and fluorescence methods is compared for several analytes. In general, peroxyoxalate chemiluminescence-based methods are 10 to 100 times more sensitive than their fluorescence-based counterparts. The chief limitation of chemiluminescence is that chemical excitation of the fluorophore apparently depends on its structure and oxidation potential. 

Mansouri A, Makris DP and Kefalas P. 2005. Determination of hydrogen peroxide scavenging activity of cinnamic and benzoic acids employing a highly sensitive peroxyoxalate chemiluminescence-based assay structure-activity relationships. J Pharm Biomed Anal 39(l-2) 22-26. 

Hexanal, hpid oxidation assessment, 669 1-Hexene, primary ozonide, 720 High-density lipoprotein (HDL) oxidation, 612 TEARS assay, 667 High-energy intermediate (HEI) chemiluminescence, 1215 activators, 1220, 1222 peroxyoxalates, 1188-9, 1257, 1261-6, 1267, 1269 stmcture, 1262, 1263... 

Among different coumarin derivatives used, 7-Amino-4-trifluoromethylcoumarin (ATFMC) revealed the most promising characteristics as an efficient fluorescent emitter. AFTMC is used in the synthesis of a substrate for fluorimetric assay of proteolytic enzymes and for use as a laser dye. We have recently investigated the chemiluminescence reactions of some peroxyoxalate esters, hydrogen peroxide and AFTMC. In this paper we report the solvent effects on the kinetics of the chemiluminescence process of the peroxyoxalate chemiluminescence in the presence of AFTMC. 

Oh SK, Cha SH. Effect of sodium azide on peroxyoxalate chemiluminescence assay method. Anal Biochem 1994 218 222-4. 

This brief summary of peroxyoxalate chemiluminescence in no way does justice to the topic and the interested reader is urged to consult the more comprehensive accounts cited in this chapter. If and when water-soluble oxalate esters become readily available, there is no doubt that these labels will be adapted to the types of assay that have formed the main subject matter for this article, viz., immunoassays, nucleic acid and gene probe assays, and clinical chemistry assays. 

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