Bacterial luminescence inhibition assay

Vibrio fischeri, bacterial luminescence inhibition test or Microtox assay (DEVL34, 1998). 

Bacterial luminescence inhibition assay on Vibrio fischeri (formerly Photobacterium phosphoreum). 

Aqueous extracts of cat s claw were tested for cytotoxicity in four in vitro bioassays using Chinese hamster ovary cells and bacterial cells (Santa Maria et al., 1997). Concentrations of 10, 20, 30, 40, 50, 75, and 100 mg/mL were used. The neutral protein assay (measures inhibition of cell growth), the total protein content assay, the tetrazolium assay (measures mitochondrial succinic dehydrogenase activity), and the microtox assay (measures inhibition of light output from a luminescent bacterium) showed no evidence of cytotoxicity. 

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