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Asparaginyl-tRNA synthetase

Direct Pathway Inhibitors of Glutaminyl-tRNA Synthetase and Asparaginyl-tRNA Synthetase... [Pg.417]

More than one-third of the ribose interactions in ATP, ADP, and FAD-protein complexes are mediated by water linking from the 2 and/or 3 hydroxyls to the protein (19). Although water-mediated links can be used to broaden ligand specificity, they may also reduce it, as in the water-assisted asparagine recognition by asparaginyl-tRNA synthetase (Fig. 2) (20). [Pg.1997]

Iwasaki W, Sekine S-1, Kuroishi C, Kuramitsu S, Shirouzu M, Yokoyama S. Structural basis of the water-assisted asparagine recognition by asparaginyl-tRNA synthetase. J. Mol. Biol. 2006 360 329-342. [Pg.2000]

Asparaginyl-tRNA synthetase SLIDE [63, 64] Single-structure-based docking Discovery of new ligands pM [65]... [Pg.251]

Discovering new classes of Brugia malayi asparaginyl-tRNA synthetase... [Pg.261]

AsnRS was first characterized in the early 1960s in L. arabimsus hy its distinct chromatographic properties from aspartyl-tRNA synthetase and Asn synthetase and, at the end of the decade in E. coli as a protein of 90-100 kDa able to aminoacylate tRNA but not tRNA f / ° The enzyme was further characterized in mitochondria from Neurospora crassa" and in rat liver where tRNA asparaginylation activity was found associated with proteins of Air 35 and 90 kDa. The AsnRS purified from B. stearothermophilus of 127 kDa was characterized as a homodimer az (a = 51 kDa).AsnRSs isolated until now from various prokaryotic and eukaryotic organisms show conservation of the homodimeric structure. Investigation of B. stearothermophilus AsnRS allowed the determination of its physicochemical parameters S2o a = 6.6 x 10 s. [Pg.397]

Figure 7 The direct and indirect pathways of tRNA asparaginylation. The direct pathway consists of charging by AsnRS on tRNA " of free Asn formed with asparagine synthetase A or B. The Asn-tRNA " binds the EF-Tu factor in bacteria (or EF-1A in eukaryotes and archaea) to be carried to the ribosome, in the indirect pathway, a nondiscriminating AspRS (ND-AspRS) charges Asp on tRNA " Asp-tRNA " does not bind the eiongation factor but is converted by the tRNA-dependent trimeric amidotransferase GatCAB into Asn-tRNA ", which binds the EF-Tu factor and is carried to the ribosome where it is used for polypeptide chain elongation. Figure 7 The direct and indirect pathways of tRNA asparaginylation. The direct pathway consists of charging by AsnRS on tRNA " of free Asn formed with asparagine synthetase A or B. The Asn-tRNA " binds the EF-Tu factor in bacteria (or EF-1A in eukaryotes and archaea) to be carried to the ribosome, in the indirect pathway, a nondiscriminating AspRS (ND-AspRS) charges Asp on tRNA " Asp-tRNA " does not bind the eiongation factor but is converted by the tRNA-dependent trimeric amidotransferase GatCAB into Asn-tRNA ", which binds the EF-Tu factor and is carried to the ribosome where it is used for polypeptide chain elongation.
See other pages where Asparaginyl-tRNA synthetase is mentioned: [Pg.383]    [Pg.384]    [Pg.400]    [Pg.423]    [Pg.35]    [Pg.1998]    [Pg.253]    [Pg.508]    [Pg.383]    [Pg.384]    [Pg.400]    [Pg.423]    [Pg.35]    [Pg.1998]    [Pg.253]    [Pg.508]    [Pg.385]   
See also in sourсe #XX -- [ Pg.253 ]



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