Asparaginase bacterial

Marlborough, D.I., Miller, D.S. Cammack, K.A. (1975). Comparative study on conformational stability and sub-unit interactions of two bacterial asparaginases. Biochimica Biophysica Acta, 386, 576-89. 

Tsuji (84) was the first to report the presence of L-asparaginase in E. coli extracts. In 1964, Mashbum and Wriston (9) described a partial purification of the enzyme from E. coli B, and found that this bacterial asparaginase possessed antilymphoma activity. Purification to apparent homogeneity was reported in 1968 (61). Roberts et al. (48) in that same year also described an extensive purification in high yield of E. coli... 

Aghaiypour, K., Wlodawer, A. and Lubkowski, J. (2001a). Do bacterial 1-asparaginases utilize a catalytic triad Thr-Tyr-Glu Biochim. Biophys. Acta 1550, 117-128. 

An enzyme of mammalian origin would almost certainly be more effective than an enzyme of bacterial origin for the treatment of tumours. However, for clinical use bacterial enzymes must be used because of the quantities involved a normal course of treatment for leukaemia requires 200-1000 units of asparaginase/ kg/day for 30 days (i.e. 10 imits). There are 3 units/ml of asparaginase in guinea-pig serum ... 

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