Aryl-0-glucosidase activity

Figure 9. Enzyme production by T. reesei QM 9414 incubated in the presence of ImM sophorose. The incubation medium (27) included 17mM potassium phosphate buffer, pH 6.0, at 28°C. The appearance of aryl-p-D-glucosidase activity (A) in the extracellular medium is delayed in comparison to endoglucanase activity (O) and Avicelase activity ( ).

For the last few years, we have investigated the cellulase components of Pseudomonas fluorescens var. cellulosa in special regard to the physiological relation of their synthesis and localization to the cultural conditions. This pseudomonad is an aerobic mesophilic cellulose-decomposing bacterium isolated earlier by Ueda et al. (39) from field soil. Some of the enzymatic properties of cellulases obtained from it have previously been reported (28). In the present review, the results of our recent studies (42) are described and discussed together with the related works of other authors. [In these studies, activities of cellulase and aryl / -glucosidase were assayed by the same methods as described in a recent paper (31), and that of amylase by the blue value method modified by Fuwa (10)]. 

The enzymes cellulase, mannanase, and aryl-/ -glucosidase showed no significant decrease in activity as determined in the presence of up to 60% of ethanediol. Xylanase, / -glucosidase, and mannosidase lost approximately half of their initial activity at this ethanediol concentration while xylosidase lost most of its activity. The inhibition caused by ethanediol was found to be reversible. 

Levels of D-glucosyl ceramidase in mice dropped very rapidly after conduritol B epoxide injection, reaching a minimum after 1 hr in liver.There was a noticeable restoration of activity within the next 2-3 hr. The level of aryl /3-D-glucosidase decreased somewhat less than that of cerebrosidase, reaching a minimum within 2 h it too showed some recovery of activity within 3 hr. 

Fig. 1. Cultivation of A. terreus ATG-5 in mineral medium supplemented with 1% cellulose, a) growth of cells and production of extracellular and bound activity of FP-cellulase (FPA) b) production of extracellular and bound activity of endoglucanase (CMCA) c) production of extracellular and bound activity of aryl-3-glucosidase (3GA). (1) extracellular enzymes (2) bound enzymes ... 

Effect of temperature on activity of FP-cellulase (FPA 1), endoglucanase (CMCA 2) and aryl-B-glucosidase (3GA 3) of A. terreus ATG5. 

Fig. 7. Effect of fed batch (source of carbon) and double fed batch (source of carbon and source of nitrogen) cultivation on the production of aryl-3-glucosidase in A. terreus ATG-5. ( > ) extracellular enzyme activity in fed batch (2,a) extracellular enzyme activity in double fed batch (3,o) specific enzyme activity referred to medium protein in fed batch (4> ) specific enzyme activity in double fed batch.

A jS-D-glucosidase that is active towards aryl /S-D-glucopyranosides has been identified in the aquatic fungus Lagenidium giganteum, a parasite of mosquito larvae. ... 

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