Arabidopsis transgenic plants

Fig. 2. Accumulation of PHA inclusions in Arabidopsis transgenic plants. Transgenic cell expressing the poly(3HB) pathway in the plastid showing accumulation of poly(3HB) inclusions (arrows) in the chloroplast of a leaf mesophyll cell. Bar represents 1 pm... 

Figure 8,15. A. Localization of T-ONA integration in the genome of transformed Arabidopsis. Transgenic plants were obtained by transformation with Agriihacieriian plasmids, which contained a uidA (C.ilJS) reporter gene. A GUS probe was hybridized on CsiSO,7KAMI) DNA fractions restricted with /f//tdlll, ail enzyme not cutting the GUS sequence. Arabidopsis DNA was pooled from 30 plants, P, pellet 1-12. DNA fractions. B. i lybridization of GUS sequence on Cs SO, /BAMD fractions from rice. 1-12, ONA...

Xiang C., Werner B.L., Christensen E.M., Oliver D.J. The biological functions of glutathione revisited in Arabidopsis transgenic plants with altered glutathione levels. Plant Physiology, 126 2564-2574 (2001). 

The two-hybrid yeast system has also been used as an approach to identifying host genes involved in transformation efficiency. As mentioned previously, VirE2 has been shown to interact with VIPl in Arabidopsis plants. Overexpression of VIPl in transgenic plants significantly increased the transformation efficiency as well as the rate of transformants that can accumulate. 

Another Arabidopsis mutant, murl, which lacks the ability to synthesize 1-fucose, possesses a defective gene encoding GDP-d-Man-4,6-dehydratase, a key enzyme in 1-fucose biosynthesis. Further analysis revealed that 1-Fuc is replaced by 1-Gal, a structurally similar monosaccharide, in the cell walls of this mutant with no adverse effects on plant physiology or metabolism (Rayon et al, 1999). Transgenic plants containing this mutation can also be used for foreign protein production. 

MANGOLD, U EICHEL, J., BATSCHAUER, A., LENZ, A., KAISER, T SPANGENBERG, G., WERCK-REICHHART, D, SCHRODER, J., Gene and cDNA for plant cytochrome P450 proteins (CYP 72 family) from Catharanthus roseus and transgenic expression in tobacco and Arabidopsis thaliana., Plant Sci., 1994, 96, 129-136. 

Kim, S. A., Kwak,J. M.,Jae, S. K., Wang, M. H., and Nam, H. G. (2001). Overexpression of the AtGluR2 gene encoding an Arabidopsis homolog of mammalian glutamate receptors impairs calcium utilization and sensitivity to ionic stress in transgenic plants. Plant Cell Physiol. 42, 74-84. 

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