Aqueous biphasic systems extraction chromatography

NEW TECHNOLOGIES FOR METAL ION SEPARATIONS POLYETHYLENE GLYCOL BASED-AQUEOUS BIPHASIC SYSTEMS AND AQUEOUS BIPHASIC EXTRACTION CHROMATOGRAPHY Robin D. Rogers and Jianhua Zhang... 

New Technologies for Metal Ion Separations Polyethylene Glycol Based-Aqueous Biphasic Systems and Aqueous Biphasic Extraction Chromatography... 

Example 1. Kinetic Resolution in a Biphasic System [20]. A solution of antibody 84G3 (500 mg, 6.67 pmol active site, 0.0086 mol%) in phosphate buffered saline (PBS pH 7.4, 87.5 mL) at room temperature vas added to a solution of ( )-45 (20 g, 77.4 mmol) in toluene (600 mL) in a Teflon tube. The mixture vas shaken at 250 rpm. under argon at the same temperature and the ee vas monitored by chiral-phase HP LG. When ee > 99% vas reached (65 h) the mixture vas cooled to —20 °G for several hours to enable the aqueous phase to freeze. The organic phase vas decanted. The frozen aqueous phase vas left to thaw and extracted vith toluene (3x3 vol. equiv.) and 1 1 EtOAc-toluene (3x3 vol. equiv.). The combined organic phase vas dried over Na2S04, concentrated in vacuo, and purifled by silica gel column chromatography (1 3 EtOAc-hexane) to afford (S)-45 (10 g, 50%). 

An overview chapter by Hamel and Hunter presents the state of the art of research on bioseparations. Extraction processes using biphasic aqueous systems, liquid membranes, reversed-micellar systems, and membrane processes are all being actively studied. Significant advances in these topics, including predictive mathematical models, are presented in the first section. The second section includes several papers on affinity and other interaction techniques that are finding uses in protein purification. In the last section, we offer several reports that delineate advances in isolation and purification processes such as electrophoresis and chromatography. 

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