Aprotinin, solution preparation

Aprotinin Aprotinin should not be mixed with corticosteroids, heparin, nutrient-containing amino acids or fat emulsions, and tetracyclines. Care must be exercised when diluting solutions of colony-stimulating factors, as they become adsorbed to glass or plastic materials, and the preparation must contain a carrier protein such as albumin to avoid the losses. 

Extraction buffer 1% SDS, 1% aprotinin, 0.5 mMphenylmethylsulfonyl-fluoride (PMSF),and50mAfdithiothreitol inTris-bufferedsaline (TBS 144 mMNaCl, 25 mAfTns, pH 7.6). Aprotinin is a protease inhibitor obtainable in solution from Sigma the stock solution is diluted 1 100. PMSF, a protease inhibitor, is toxic and can be omitted in many instances. It is added from a 100 mMsolution in isoamyl alcohol. Dithiothreitol is added to the extraction buffer immediately before use. For some purposes, the assay buffer is prepared at 2X normal strength by doubling the concentrations of SDS, aprotinin, and PMSF in TBS. 

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