Approaches to Optimize the Transaminase-Catalyzed Reactions

An issue that has recently emerged was a microscale method to assay bioprocess options to improve bioconversion reactions yields by overcoming thermodynamic and kinetic limitations [110]. In this study they observed that the choice of the amino donor and the equilibrium shift via second enzyme reactions are the best options to increase the product yield. 

Nevertheless, to customize industrial needs and to overcome the deficiencies of enzyme reactions, protein engineering makes it feasible to modify enzymes for certain processes [4,68,73, 111, 112]. 

A new approach to protein engineering is computational design. This method provides a wider range of gaining new active enzymes via the access to structures. 

It is apparent that computational design improves the chances of finding active enzymes within a clone library yet the variety of the catalyzed reactions by the designed enzymes is still restricted [113]. The latest achievements in protein engineering have been reviewed extensively [114-116]. 

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