Applied Biosystems 4700 Proteomics Analyzer

Several commercial instrument designs have effectively overcome this inherent problem and can record high-energy CID product ion spectra. The Applied Biosystems 4700 Proteomics Analyzer was one of the two originally available commercial TOF/TOF designs76,77 (Fig. 24). When the instrument is operated to obtain a mass spectrum, it acts as a standard inline reflectron TOF instrument. In product ion experiments the precursor ion is isolated at the end of the first flight tube by the timed-ion selectors (TIS). The MALDI source uses DE to correct for... [Pg.92]

Another technological development in tandem mass spectrometry is the combination of two TOP mass spectrometers, TOF/TOK These instruments have excellent sensitivity and throughput for MALDI-MS/MS and are especially suited for proteomics research. However, these instruments are unable to perform true precursor ion scans or constant neutral loss scans. The Bruker Daltonics ultra-flex and Applied Biosystem 4700 proteomics analyzer are examples of this type of instrument, Such mstruments have yet to make an impact in clinical chemistry. [Pg.180]

MALDI-TOF/TOF-MS (4700 Proteomics Analyzer, Applied Biosystems Applera... [Pg.33]

The acquisition of the mass spectra for peptide and protein imaging is done in our lab on commercial MALDI TOF instruments (Voyager sSTR, 4700 Proteomics Analyzer Applied Biosystems, Framingham, MA) equipped with a Nd YAG laser. The acquisition of the data is controlled by an in-house designed software (MALDI MS Imaging Tool, MMSIT). The area to be scanned is specified as a pattern of points, equidistant from each other. The distance is chosen referring to the diameter of the... [Pg.330]

Proteome-Analyzer 4700 (Applied Biosystems, Foster City, CA, USA). [Pg.30]

Carry out MALDI-TOF MS analyses of spotted peptide solutions on a Proteome-Analyzer 4700 (Applied Biosystems). The spectra are recorded in a reflector mode in a mass range from 900 to 3700 Da. For one main spectrum, 25 subspectra with 100 spots per subspectrum are accumulated using a random search pattern. If the autolytical fragment of trypsin with the monoisotopic (M+H)+ m/z at 2211.104 reaches a signal-to-noise ratio (S/N) of at least 10, an internal calibration is automatically performed using the peak for one point calibration. The peptide search tolerance is 50 ppm, but the actual standard deviation is between 10 and 20 ppm. [Pg.39]

Bacillus anthracis, Bacillus cereus P I,E CHCA Data Explorer Applied Biosystems Ultraflex I, Bruker Daltonics 4700 Proteomics Analyzer TOE-TOE Applied Biosystems Casfanha ef al. 2007... [Pg.280]

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