Applied Biosystems peptide synthesizer

Add solution to resin, add some DCM, and agitate for 40 min. In some protocols HOBt-activation is used as a routine (e.g., Applied Biosystems Peptide Synthesizer). 

Applied Biosystems Peptide Synthesizer User Bulletin, No. 3, August, 1985 also B. J. Bergot, R. L. Noble and T. Gerser, Op. Cit., No. 16, September, 1986. 

Different instruments are now available for automated synthesis on solid phase. The Applied Biosystems peptide synthesizer ABl 433 (Fig. 3) was chosen as an initial platform due to its wide availability and the few modifications necessary for its application to oligosaccharide synthesis. The custom-made reaction vessel is constructed of jacketed glass to allow cooling. The building blocks are placed in cartridges to be delivered sequentially. The synthesizer allows for nine different solvents and reagents. A new, more versatile and less... 

PE Applied Biosystems Peptide Synthesis Technical Bulletin. Feedback monitoring kit for ABI431A peptide synthesizer. 

Hirulog-8 has the formula H-(D-Phe)-Pro-Arg-Pro-(Gly)4-Asn-Gly-Asp-Phe-Glu-Glu-Ile-Pro-Glu-Glu-Tyr-Leu-OH. Hirulog-8 was synthesized by conventional solid-phase peptide synthesis employing an Applied Biosystems 430 A Peptide Synthesizer. This peptide was synthesized using BOC-L-Leucine-O-divinylbenzene resin. Additional t-BOC-amino acids (Peninsula Laboratories,... 

The synthesis of a linear precursor peptide of the HSV-a-[Tyr ]-conotoxin chimera (see Note 8) was performed by solid phase methodology on an Applied Biosystems Mod. 431A peptide synthesizer (Applied Biosystems Paris, France). Fmoc-based chemistry was used for the preparation of peptide using Rink-amide resin with 0.43 mmol/g loading. Four equivalents of Fmoc amino acid derivatives and HBTU/HOBt were used for coupling. The following amino acid derivatives were used in the synthesis Fmoc-Cys(Trt)-OH, Fmoc-Gly-OH, Fmoc-Val-OH, Fmoc-Pro-OH, Fmoc-Cys(Acm)-OH, Fmoc-Asn(Trt)-OH, Fmoc-Tyr( Bu)-OH. The synthetic protocol for Fmoc chemistry was as follows ... 

Transport peptides can be synthesized using either t-Boc or Fmoc solid phase peptide synthesis strategies with a synthesizer or manually. We routinely synthesize CPPs in a stepwise manner on solid support using an Applied Biosystems Model 431A peptide synthesizer. tert-Butyloxycarbonyl amino acids are coupled as 1-hydroxybenzotriazole (HOBt) esters to a p-methylbenzylhydryl-amine (MBHA) resin (65). C-terminally amidated CPPs are less prone to degradation and show higher internalization efficiency than carboxylic acid derivatives. 

Peptides were synthesized on an Applied Biosystems 430A peptide synthesizer and purified by reverse phase HPLC on a C18 column (WF peptide) or a C8 column (FFW peptide) and were provided with free carboxy and amino termini. All concentrations were determined spectrophotometrically using a calculated extinction coefficient of 5560 M" cm at 280 nm for the peptides (2 Phe and 1 Trp) and published extinction coefficients for wildtype and the four mutant calmodulins (3). 

Beginning with either Boc-Lys (CL-Z)-PAM or Boc-Orn (CL-Z)-PAM resin, the following 10-residue linear peptides were synthesized using an Applied Biosystems 430A automated peptide synthesizer. 

The quantitative ninhydrin test has not been automated. The Applied Biosystems Models 430, 431, and 433 Peptide Synthesizers, however, have the capability to automatically remove 3-10 mg resin samples after each coupling for a manual ninhydrin test. 

Peptide synthesizers with conductivity monitoring include the Applied Biosystems Synergy and Model 433A, Biotech BT 7600, and Kem-En-Tec MKIII. 

Model 430A Peptide Synthesizer Users Manual, Version 1.3B, Applied Biosystems Foster City, (1988) p 6-32. 

Peptides were synthesized according to the C-terminal sequences of human AChE-S and BChE (SwissProt accession nos. P06276 and P22303, respectively) using a Pioneer or a 433A peptide synthesizer (Applied Biosystems). Purification (>90%) by reverse-phase HPLC was followed by MALDl-TOF mass spectrometry. 

Synthesis of the four-helix bundles was accomplished by a two-step procedure (outlined as a flow chart in Figure 2). Proteins were synthesized by solid phase methods in accordance with general principles (62-64) using a peptide synthesizer (model 431, Applied Biosystems (ABI), Foster City, CA). L-con-figuration amino acid derivatives were used (The Peptide Institute, Osaka, Japan). The acid-labile fert-butyloxycarbonyl- (f- Boc)-protecting groups were... 

Polypeptoid synthesis reactions are carried out on automatic peptide synthesizers (ABI433A Applied Biosystems, Foster City, CA). Fmoc-rink amide resin (0.25 mmol Nova Biochem, San Diego, CA) is deprotected by treating with 20% (v/v) piperdine (ABI) in dimethylformamide (DMF Fisher Scientific, Itasca, IL) in two 15-min treatments. To assemble the polypeptoid chain, alternating cycles of bromoacetylation and amine displacement of the bromine from the A-terminal alkyl halide are performed (Figure 12.14). To bromoacetylate, the resin is vortexed for 45 min with 4.3 mL of... 

The peptide is synthesized on Symphony (Protein Technologies Inc.) and purified on a Delta-Pak CIS 15 xm lOOA column (Waters). The oligonucleotide is synthesized from 3 to 5 on Expedite (Applied BioSystems). The amine function with photocleavable linker is added in 5 before cleavage and deprotection. These steps are performed using a NH4OH 28% solution during 24 h in the dark. The amino oligonucleotide is then purified... 

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