Applications, phage display

Paschke, M. (2006) Phage display systems and their applications. Applied Microbiology and Biotechnology, 70,2-11. 

Chen, I., Choi, Y. A. and Ting, A. Y. (2007). Phage display evolution of a peptide substrate for yeast biotin ligase and application to two-color quantum dot labeling of cell surface proteins. J. Am. Chem. Soc. 129, 6619-25. 

A number of molecules in groups 2 and 3 have been identified by the differential homing capacity of phage display libraries and combination peptide libraries [71]. Biochemical strategies such as the application of 2D gel electrophoresis on protein extracts from endothelial cell surfaces have also proven useful in this respect [72]. 

Figure 10.4. Application of phage display in drug and target discovery.

Phage displayed peptide libraries have been snccessfnlly employed for the identification of peptide ligands with a variety of applications. Recent reviews describe the developments in the generation and screening of peptide libraries [6], their application in the identification of receptor ligands [32] and in drng development [33]. 

Table 10.1. Example of phage display ligands with therapeutic applications.

Hoogenboom, H.R., Overview of antibody phage-display technology and its applications. Methods Mol Biol, 2002.178 1-37. 

Phage display techniques can be used in any field where molecular biological approaches find application. It can be successfully used for epitope mapping, vaccine development, identification of proteinkinase substrates and nonpeptid ligands, selection of functional interactions of hormones, enzymes, enzyme inhibitors, mapping... 

Van Kuppevelt, T.H., Dennissen, M.A.B.A., van Venrooij, W.J., Hoet, R.M.A., Veerkamp J.H. (1998). Generation and application of type-specific anti-heparan sulfate antibodies using phage display technology. Further evidence for heparan sulfate heterogeneity in the kidney. J. Biol. Chem., 273, 12960-12966. 

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