Application to Trapping of Reactive Intermediates

The identification of the metabolites of drugs in humans and animal species forms the basis of a separate chapter in this volume. However, some studies have been carried out on metabolism of model herbicide compounds in plants and these are reviewed briefly here. 

Directly coupled HPLC-NMR-MS has been used to characterise the major metabolites of 5-trifluoromethylpyridone, which can also exist as the tautomer 2-hydroxy-5-trifluoromethylpyridine, in hydroponically grown maize plants 

In this study, the retention times of the parent compound, the TV-glucoside and the 0-malonylglucoside were identified by using 19F NMR spectroscopy and on-flow detected HPLC-NMR, with this being followed by stop-flow HPLC-NMR-MS. 

a study was made using 5-nitropyridone where the absence of a 19F NMR handle meant that it was necessary to use the information from the on-line MS results to identify the chromatographic peaks of interest 

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