Apple Rogosa medium

Apple Rogosa is a relatively simple to prepare general growth medium for LAB. As the reader will note, this basic medium is modified for specific diagnostic purposes (i.e., demonstration of homofermentative versus het-erofermentative utilization of glucose). 

The media outlined for the cultivation of lactic acid bacteria are the apple juice Rogosa medium (King and Beelman, 1986) and the tomato juice-glucose-fructose-malate medium (Izuagbe et al., 1985). These media use either apple juice or tomato juice serum to provide the so-called tomato juice factor (Section 2.3). Liver extract or concentrate has a number of vitamins that improves bacterial growth and is available from Sigma Chemical Company. Both media can be made selective against Sac-charomyces by the addition of cycloheximide. 

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