General Growth Medium for LAB

Apple Rogosa is a relatively simple to prepare general growth medium for LAB. As the reader will note, this basic medium is modified for specific diagnostic purposes (i.e., demonstration of homofermentative versus het-erofermentative utilization of glucose). 

Preparation Dilute 200 mL of apple juice (either frozen or juice without preservatives) to 1 L using distilled water. Transfer 700-800 mL of the diluted juice to a separate 1-L flask and dissolve the following  

Bring to 1 L final volume with diluted apple juice. Adjust pH to 5.5. Dispense into test tubes and sterilize by autoclaving. 

Note The above medium may be solidified by inclusion of agar at 20 g/L. 

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