Antioxidant status markers

R16. Rice-Evans, C. A., Measurement of total antioxidant activity as a marker of antioxidant status in vivo Procedures and limitations. Free Radic. Res. 33, S59—S66 (2000). 

YOUNG J F, DRAGSTED L O, HARALDSDOTTIR J, DANESHVAR B, KALL M A, LOFT S, NILSSON L, NIELSEN S E, MAYER B, SKIBSTED L H, HUYNH-BA T, HERMETTER A and SANDSTROM B (2002) Green tea extract only affects markers of oxidative status postprandially lasting antioxidant effect of flavonoid-free diet, Brit J Nutr, 87, 343-55. 

The assays most widely employed are the measurement of thiobarbituric acid-reactive species (TBARS) and the formation of conjugated dienes, markers of lipid peroxidation [31-33] the determination of advanced oxidation protein products (AOPP), a marker of protein oxidation, and of advanced glycation end-products (AGE) [34-37] the measurement of erythrocyte antioxidant potential [38]. Of particular importance is the isoprostanes determination, since these compounds are formed by the free radical catalysed peroxidation of arachidonic acid, which is independent of the cyclooxygenase enzyme, giving rise to stable compounds, measurable in urine [39]. As recently assessed in a Workshop on markers of oxidative damage and antioxidant protection [40], currently available methods for the determination of antioxidant and redox status are not yet generally suitable for routine clinical applications, essentially for the lack of standardized tests. 

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