Antigene technology

Fig. 6. Metal-crosslinking in antisense and antigene technology. The recognition of the target (sense) strand by the antisense strand is rapid and is followed by a slower irreversible metal crosslinking. 

Development of bridged nucleic acid analogs for antigene technology 04CPB1399. 

There are many possible means for quantification of the antigen—antibody reaction. Immunoassays may be classified according to the technology used for detection and quantification of the analyte being detected. 

In the preparation of classical killed or toxoid vaccines, simple process technology was used. With the advance of new vaccines, far more sophisticated process technologies are needed. The desire to reduce side effects of vaccination requires processes which will yield antigens of extreme purity. The new regulation in cGMP requires consistent production procedures, and global competition also demands that the most efficient process technology be appHed. 

The basic process technology in vaccine production consists of fermentation for the production of antigen, purification of antigen, and formulation of the final vaccine. In bacterial fermentation, technology is weU estabHshed. For viral vaccines, ceU culture is the standard procedure. Different variations of ceU line and process system are in use. For most of the Hve viral vaccine and other subunit vaccines, production is by direct infection of a ceU substrate with the vims. 

Hepatitis B vaccine is manufactured using recombinant DNA technology to express hepatitis B surface antigen (HBsAg) in yeast. This is further purified with biochemical separation techniques to produce the vaccine. The vaccines are formulated to contain 10 to 40 meg of HBsAg protein/mL. Hepatitis B vaccine is available as a single component or in combination vaccines. 

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