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Antigen monolayer-functionalize

The association of the DNP-Ab to the antigen-monolayer-functionalized electrode leads to the electrical insulation of the electrode support and to the introduction of an electron barrier at the electrode surface. Thus, in the presence of an electrically wired enzyme (e.g., ferrocene-tethered glucose oxidase), the bioelectrocatalytic current is inhibited upon the formation of... [Pg.252]

Long period gratings modified by the deposition of a self-assembled colloid monolayer of gold have been shown to enhance SRI sensitivity, which was attributed to the localized surface plasmon resonance (LSPR) of gold nanoparticles61. The system was used after functionalization of the gold nanoparticles with dini-trophenyl (DNP) antigen, for the detection of anti-(DNP) with a limit of detection as low as 9.5 x 10 10 M. [Pg.71]

Hydrazine activation can also be used as the basis for site-directed immobilization or directional immobilization [16]. Directional immobilization is the immobilization of the sensor detector molecules in a highly ordered and reproducible manner. For example, in antibody-based biosensors requiring a monolayer coating of antibody on the transducer, it is critical to sensor functionality and sensitivity that as many of the antibodies as possible are immobilized such that their antigen binding sites are directed outward and easily accessible by antigen (analyte). [Pg.211]

The use of electrodes modified with immobilized redox-active molecules provides a simple methodology by which to study the ultrathin film electrochemistry of water-insoluble redox-active molecules, encouraging the application of such techniques to biomimetic membranes in aqueous media. It is of interest to use monolayer and LB films of enzymes, proteins, and antibodies as biosensors or biomolecular switches because of their high sensitivity for their substrates and antigens, respectively. The formation of thin fullerene films including fullerene-lipid hybrid and fullerene-lipid composite bilayer membranes is of interest both from a fundamental and practical application point of view. Multiwalled carbon nanotubes (MWNTs) and single-walled carbon nanotubes (SWNTs) are novel nanomaterials that have remarkable electronic, mechanical, and thermal properties, and specific functions. Soluble carbon nanotubes in aqueous and organic systems are of interest since their study allows the... [Pg.6391]


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