Antibody glycolipids

Figure 22.15 Glycolipid components included in liposome construction may be used to couple antibody molecules by using conjugates of lectins with the proper specificity for binding the sugar groups.

Willison, H. J. and Yuki, N. Peripheral neuropathies and anti-glycolipid antibodies. Brain 125 2591-2625, 2002. 

Once the monoclonal antibodies reacting with complex glycolipids were shown to occur frequently in patients with neuropathy in association with gammopathy, antibodies were demonstrated in some patients with GBS and other forms of inflammatory demyelinating neuropathy [21]. 

Research has resulted in major advances in animal modeling of anti-glycolipid antibody-associated neuropathies in vitro and in vivo, which make a strong case that the antibodies cause much of the neurological impairment in these patients [21]. Furthermore, molecular... 

Sugars, glycoproteins, and glycolipids Complementary nucleic acids and DNA-/RNA-binding proteins Antibodies... 

Mcllhinney, R. A. J., Patel, S. and Gore, M. E. 1985. Monoclonal antibodies recognizing epitopes carried on both glycolipids and glycoproteins of the human milk fat globule membrane. Biochem. J. 227, 155-162. 

Yanagisawa, M., Kojima, H., Kawakami, Y., Iriko, H., Nakamura, T., Nakamura, K., Uchida, A., Murata, Y. and Tamai, Y. (1 999) A monoclonal antibody against a glycolipid SEGLx from Spirometra erinaceieuropaei plerocercoid. Molecular and Biochemical Parasitology 102, 225-235. 

The synthetic pentasaccharide showed similar antigenic specificity to the Forssman glycolipid but because of the absence of the lipid portion did not form a stable complex with the Forssman antibody [187],... 

Numerous studies of specificities of anti-glycolipid antibodies produced by immunizing rabbits have been reported (8,9), and some discrepancies have been noted between laboratories (9). The widespread occurrence of natural antibodies might cause confusion in analyzing specificities produced by experimental immunization. Because of this we have purified both immune and natural anti-glycolipid antibodies from rabbit sera, and we have compared their specifities against the same, and different, glycolipids. 

Figure 1. Natural antibodies in normal human and rabbit sera against liposomes containing no glycolipid, galactosylceramide, or lactosylceramide. Glucose release measured from liposomes containing DMPC, CHOL, DCP, and, where indicated, galactosylceramide (150 fig/nmol PC) or lactosylceramide (150 ng/fimol PC).

TABLE II. HUMAN NATURAL ANTIBODIES AGAINST GLYCOLIPIDS HAVING TERMINAL N-ACETYLGALACTOSAMINE RESIDUES... 

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