Glycolipid against

Wiels, J., Junqua, S., Dujardin, P., Le Pecq, J.B., and Tursz, T. (1984) Properties of immunotoxins against a glycolipid antigen associated with Burkitt s lymphoma. Cancer Res. 44, 129. 

Yanagisawa, M., Kojima, H., Kawakami, Y., Iriko, H., Nakamura, T., Nakamura, K., Uchida, A., Murata, Y. and Tamai, Y. (1 999) A monoclonal antibody against a glycolipid SEGLx from Spirometra erinaceieuropaei plerocercoid. Molecular and Biochemical Parasitology 102, 225-235. 

The major purpose of the present study was to describe, and to quantify, the widespread occurrence of natural complement-fixing autoantibodies against numerous simple glycolipids. We show that every individual rabbit and human serum tested had complement-fixing autoantibodies against glycolipids that are widely distributed in circulating blood cells and other tissues. 

Numerous studies of specificities of anti-glycolipid antibodies produced by immunizing rabbits have been reported (8,9), and some discrepancies have been noted between laboratories (9). The widespread occurrence of natural antibodies might cause confusion in analyzing specificities produced by experimental immunization. Because of this we have purified both immune and natural anti-glycolipid antibodies from rabbit sera, and we have compared their specifities against the same, and different, glycolipids. 

Figure 1. Natural antibodies in normal human and rabbit sera against liposomes containing no glycolipid, galactosylceramide, or lactosylceramide. Glucose release measured from liposomes containing DMPC, CHOL, DCP, and, where indicated, galactosylceramide (150 fig/nmol PC) or lactosylceramide (150 ng/fimol PC).

TABLE II. HUMAN NATURAL ANTIBODIES AGAINST GLYCOLIPIDS HAVING TERMINAL N-ACETYLGALACTOSAMINE RESIDUES... 

We have demonstrated that every one of the human and rabbit sera tested have natural antibodies against several glycolipids. Most of the glycolipids used as antigens in this study normally occur in human and rabbit tissues (18), and therefore many, if not all, of the activities described are due to autoantibodies. 

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