Antibody-bearing liposomes

Agrawal, A, K., Singhal, A., and Gupta, C, M. (1987). Functional targeting to erythrocytes in vivo using antibody bearing liposomes as drug vehicles, Biochem. Biophys. Res. Commun., 148, 357-361. 

Covalent attachment of antibody molecules to liposomes can provide a targeting capacity to the vesicle that can modulate its binding to specific antigenic determinants on cells or to molecules in solution. Antibody-bearing liposomes may possess encapsulated components that can be used for detection or therapy (Figure 22.17). For instance, fluorescent molecules encapsulated within antibody-targeted vesicles can be used as imaging tools or in flow cytometry... 

Kung, V.T., Maxim, P.E., Veltri, R.W., and Martin, F.J. (1985) Antibody-bearing liposomes improve agglutination of latex particles used in clinical diagnostic assays. Biochim. Biophys. Acta 839, 105-109. 

Truneh, A., Machy, P., and Horan, P.K. (1987) Antibody-bearing liposomes as multicolor immunofluores-cent markers for flow cytometry and imaging./. Immunol. Meth. 100, 59-71. 

The NHS ester reacts with amine groups on the protein molecule, producing stable amide bond linkages (Fig. 349). The specificity of the antibody-bearing liposomes for mouse L-929 cells was documented, illustrating the preservation of antibody-binding activity. 

Antibody-Bearing Liposomes as Chloroquine Vehicles in Treatment of Murine Malaria... 

Since protein absorbance interferes with accurately determining the amount of chloroquine entrapped in antibody-bearing liposomes, i.e., the chloroquine should be measured prior to the liposome coupling to F(ab )2 fragments. 

G. V. Betageri and L. S. Burrell, Stability of antibody-bearing liposomes containing dideoxyinosine triphosphate, Int. J. Pharm. 98, 149-155(1993). 

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