Antibodies immuno-electron microscopy

Because of the high specificity of an antibody for its epitope, an antibody raised against a particular protein antigen can be used to determine the location of that antigen in a cell using immunofluorescence light microscopy or immuno-electron microscopy. 

Antibodies against sugars (carbohydrate residues) can be difficult to obtain and lectins are a solution to these problems. Lectins are naturally occurring plant and animal proteins or glycoproteins that selectively bind noncovalently to carbohydrate residues. Lectins can be labeled directly or secondary antibodies against lectins enables the use of other immuno techniques (30) including electron microscopy (31). 

Transmission electron microscopy analyses of ultrathin sections have indicated that there are several vacuoles in myrosin cells of embryos. These vacuoles contain myrosinase, as revealed by immunogold labelling studies, and have been referred to as myrosin grains.40, 1 Recently, confocal laser scanning immuno-microscopy analysis with the anti-myrosinase monoclonal antibody 3D7 has been... 

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