Antibodies, hydrogen peroxide determination

Antarctic air, hydrogen peroxide determination, 648 Anthocyanins, TEARS assay, 667 Anthracene, primary ozonides, 723-4 Anthropogenic emissions atmosphere, 604, 605 hydrogen peroxide, 626 Antibodies, hydrogen peroxide determination, 1315... 

Figure 3.29.A shows a flow-cell of 20 iL inner volume used to hold immobilized anti-mouse IgG bound to a rigid beaded support (activated Pierce trisacryl GF-2000). The cell was used to develop a two-site immunoassay for mouse IgG by consecutive injection of the sample, acridinium ester-labelled antibody and alkaline hydrogen peroxide to initiate the chemiluminescence, which started the reaction sequence shown in Fig. 3.29.B. Regenerating the sensor entailed subsequent injection of an acid solution, which resulted in a determination time of ca. 12 min (this varied as a fimction of the flow-rate used, which also determined the detection limit achieved, viz. 50 amol for an overall analysis time of 18 min) [218]. The sensor was used for at least one week with an inter-assay RSD of 5.9%. Attempts at automating the hydrodynamic system for use in routine analyses are currently under way.

Chemiluminescence immunoassay These assays rely on the use of chemiluminescent labels, which may be labelled antigens or antibodies. For example, the chemiluminescent label isoluminol is oxidized in the presence of hydrogen peroxide and a catalyst, producing long-lived light emission that is measured, thus allowing determination of the unknown antigen or antibody concentration in a sample. Another useful variant is electrochemiluminescence immunoassay that is commonly used in biomolecular detection, and in particular the measurement of native and recombinant peptides and proteins. 

Xu Q, Zhu JJ, Hu XY (2007) Ordered mesoporous polyanifine film as a new matrix for enzyme immobilization and biosensor construction. Anal Chun Acta 597(1) 151—156 Yakovleva J, Davidsson R, Lobanova A et ak (2002) Enzyme immunoassay using silicon microchip with immobilized antibodies and chemiluminescence detection. Antd Chem 74(13) 2994—3004 Yamada H, Kobayashi M (1996) Nitrile hydiatase and its application to industrial production of acrylamide. Biosci Biotechnol Biochem 60 1391—1400 Yamamoto K, Ohgam T, Torimura M et al. (2000) Highly-sensitive flow injection determination of hydrogen peroxide with a peroxidase-immobilized electrode and its application to clinical chemistry. Anal Chim Acta 40(2) 201—207... 

Figure. 3 Sequential injection electrochemical immunoassay system for determination ofTCP.and TCP-HRP conjugates for competitive imrnunoreaction and the substrate solution containing hydrogen peroxide is sent for electrochemical detection. Antibody-modified magnetic beads in the tube were used as a platform for competitive imrnunoreaction.

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