Production Strains Devoid of Antibiotic Resistance Markers

Production Strains Devoid of Antibiotic Resistance Markers [Pg.238]

Chromosomal Modification with Transiently Employed Selection Markers [Pg.238]

The resulting transformants are antibiotic sensitive, which is a convenient screening criterion for correct chromosomal integration. The method has been used to replace native, regulated promoters with strong constitutive promoters for genes and operons involved in the biosynthesis of riboflavin [135], biotin [176], pantothenate [134,177], folate, and thiamin [178]. This method can also be used to introduce point mutations, as long as sufficient enzymatic activity remains to allow for selection of prototrophic colonies [179,180]. [Pg.239]

Counterselectlon Strategies to Force the Deletion of Dominant Markers [Pg.239]

To the authors of this chapter, it is unclear to what extent the elaborate marker replacement strategies outlined above have found their way into the reality of industrial production strain development. [Pg.240]

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