Angiogenesis Quantitative methods

Passaniti, A., Taylor, R. M., Pih, R., Guo, Y., Long, P. V, and Haney, J. A. (1992). A simple, quantitative method for assessing angiogenesis and antiangiogenic agents using reconstituted basement membrane, heparin, and fibroblast growth factor. Lab. Invest. 67, 519-528. 

There are several in vivo assay models for angiogenesis, including the shell-less chicken chorioallantoic membrane (CAM) assay, the cornea assay, and the endothelial cell assay. The CAM assay is most frequently used assay method because it is easy to use and less costly. In this assay, an inhibitor s effect on the shell-less chicken embryo culture can be observed directly, although quantitating the result is subjective. The mouse cornea assay is widely favored because the cornea is an avascular tissue, any blood vessel development unequivocally demonstrates the lack of antiangiogensis activity [77-79]. However, all of these in vivo assay... 

Grischke EM, Kaufmann M, Eberlein-Gonska M, Mattfeld T, Sohn Ch, Bastert G (1994) Angiogenesis as a diagnostic factor in primary breast cancer Microvessel quantitation by stereological methods and correlation with color Doppler sonography. Onkologie 17 35-42... 

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