Analytical schlieren patterns

Figure 6. Model E analytical schlieren patterns of purified LPS. Equilibrium attained at 36,000 rpm at 25°C for 18 hours in 2°C sector cells. A 34% CsCl pattern of B. abortus LPS, sample from preparation run in Figure 5A. B 34% CsCl pattern of B. abortus LPS. C 39% CsCl pattern of P. multocida LPS.

Mixing conditions. In a flow analyser comprising a mixing chamber e.g., for analyte separation, a Schlieren pattern is observed inside the chamber inner volume under poor mixing conditions. This effect alters the recorded peak profile, which becomes noisy and less reproducible. 

Other optical techniques have also been used to monitor analytical ultracentrifugation experiments. These included refractive index (Schlieren optics) as well as interference patterns produced using Rayleigh- or Lebedev-type optics.11 In both cases, data are acquired using photographic or video recording equipment. Examples of data obtained with these different optical systems are shown in Figure 13.11. 

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