Analytical Methods for Detection of A9-THC and Other Cannabinoids in Plants

Analytical Methods for Detection of A9-THC and Other Cannabinoids in Plants [Pg.25]

Many methods for determination of cannabinoids in plant material have been developed. Commonly HPLC or GC is used, often in combination with mass spectrometry. Molecular techniques are also available to detect these compounds and will be discussed in this section. [Pg.25]

Usually the first step is an extraction of the desired compounds from plant material. This extraction can be done by different solvents, e.g., methanol [85], n-hexane [86], petroleum ether or solvent mixtures such as methanol/chloroform [87]. The use of a second liquid-liquid extraction (LLE) with 0.1 M NaOH after extraction with a non-polar solvent like n-hexane makes a separate analysis of acidic cannabinoids possible, which can be found [Pg.25]

In comparison to GC, an advantage in using HPLC is that there is no decomposition of the acidic forms of cannabinoids. Commonly reversed-phased (RP) materials are used as the stationary phase. Mostly the octadecyl-type [Pg.26]

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