Analytical methods amplification

It has been established, that both DN and Ibp form complex compounds with ions Eu(III), Sm(III), Tb(III) and Dy(III), possessing luminescent properties. The most intensive luminescence is observed for complex compounds with ion Tb(III). It has been shown, that complexation has place in low acidic and neutral water solutions at pH 6,4-7,0. From the data of luminescence intensity for the complex the ratio of component Tb Fig was established equal to 1 2 by the continuous variations method. Presence at a solution of organic bases 2,2 -bipyridil, (Bipy) and 1,10-phenanthroline (Phen) causes the analytical signal amplification up to 250 (75) times as a result of the Bipy (Phen) inclusion in inner coordination sphere and formation of different ligands complexes with component ratio Tb Fig Bipy (Phen) = 1 2 1. 

In our research, we proposed a post amplification analytical method to detect F. culmorum, a pathogen causing foot rot and head blight diseases in cereals, and can produce mycotoxins such as zearalenone, deoxynivalenol, and other trichothecenes that can enter the food chain. The early identification of this fungal pathogen is therefore recommended in order to avoid crop losses and protect consumer health... 

The use of europium chelates, with their unusually long fluorescence decay times, as labels for proteins and antibodies has provided techniques that are referred to as time-resolved fluoroimmunoassays (TRFIA). Fluorophores as labels for biomolecules will be the topic of Sect. 3. Nevertheless, TRFIAs always have to compete with ELISA (enzyme-linked immunosorbent assays) techniques, which are characterized by their great versatility and sensitivity through an enzyme-driven signal amplification. Numerous studies have been published over the past two decades which compare both analytical methods, e.g., with respect to the detection of influenza viruses or HIV-1 specific IgA antibodies [117,118]. Lanthanide luminescence detection is another new development, and Tb(III) complexes have been applied, for instance, as indicators for peroxidase-catalyzed dimerization products in ELISAs [119]. 

Mass spectrometry is an analytical method of choice for identification of volatile compounds and has been employed in investigation of thermolysis and acidolysis mechanisms of chemical amplification resists [96, 121, 122]. This technique has been also utilized in screening of resists systems, especially Si-containing 193 nm bilayer resists,for outgassing [438,439]. MALDI-TOF mass spectroscopy has been applied to characterization of dendritic resist polymers. 

A number of changes are necessary to adapt this definition to the present state of analytical methods, not only in inorganic and organic analysis but also in biochemical analysis. However, two basic ideas must be retained the fact that the use of amplification reactions is a way of increasing the sensitivity of the analytical measurements, and that these reactions can be carried out in an iterative form. 

DNA detection offers several ways of interfacing DNA recognition events with electrochemical signal transduction. Among the many established methods, amplification is one of the most important concepts because it permits a highest analytical sensitivity. Some of the most widely used methods applied in DNA sensors such as a voltammetric, potentiometric. 

Recently chemical amplification type photoresists have been developed. In the present paper, we described a novel analytic method of photoadd generation. This method is very simple and useful for investigation of photoadd generators. 

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