Electrophoresis bands

From the beginning of free solution electrophoresis, band broadening from thermal effects was foreseen as the main problem restricting its development [6]. The energy generated as heat by application of an electric field to an electrolyte solution in a column raises the solution temperature, but more critically, results in a radial... 

In electrophoresis, band broadening is mainly caused by longitudinal diffusion. The peak dispersion, o, is directly proportional to the diffusion coefficient, D, of the analyte and its migration time, t ... 

In theory, millions of theoretical plates per metre can be achieved with electrophoresis making this technique superior to LC, where the number of plates per column is typically in the order of tens of thousands (section 2.2). In practice, however, lower plate numbers are observed in electrophoresis. Band broadening is... 

The reaction of l-ethyl-3(3-dimethylaminopropyl)carbodi-imide with bovine lutrophin has been studied and the reaction conditions have been investigated. Spectrophotometric measurements, tryptic peptide maps, and sodium dodecyl sulphate-urea polyacrylamide gel electrophoresis banding patterns were obtained and compared for native and cross-linked derivatives of the glycoprotein hormone. ... 

UHMWPE samples were incubated in BSF, and investigated for adsorbed proteins in the range of plasma proteins by means of protein desorption and gel electrophoresis. Bands of adsorbed proteins corresponding to albumin, immunoglobulin heavy chain... 

Fig. 9.3 Dendrogram derived from clustering analysis of denaturating gel electrophoresis banding profile of rumen bacterial 16S rRNA from lambs fed concentrate with (TY) or without (TN) tannins...

Due to the insolubility of AP-aggregates, fibril formation could not be analyzed directly by mass spectrometry. In order to determine the molecular composition of aggregates, in gel tryptic digestion and mass spectrometric analysis of the gel electrophoresis bands of Ap-oligomers was performed. AP(l-40) was subjected to fibril growth at 1 J,g/ jL (220 pM) in buffer solution, pH 7.5, and incubated for 5 days at... 

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