Analysis of serum lipoproteins by HPLC

ANALYSIS OF SERUM LIPOPROTEINS BY HPLC 4.1. Detection of protein moiety 

As described in Section 3.4, the elution patterns of serum lipoproteins can be obtained by monitoring A gg with use of the total lipoprotein fraction prepared from serum by ultracentrifugation. A few examples obtained for individual human subjects are shown in Fig. 9. The level of each lipoprotein is found to vary with individual subjects. Especially in the case of pathological subjects, there are distinct patterns with respect to each disease. 

Although the separation profile of the total lipoprotein fraction can be given by monitoring of protein moiety at A gg, the concentration of each lipoprotein class could not be calculated from peak area because of the difficulties in estimating lipoprotein levels caused by the following factors evaluation of turbidity, different molar coefficient of A2gg due to each lipoprotein, coelution of serum proteins with HDL fractions. At this point of view, a selective detection method of lipid components by enzymatic reaction using whole serum is useful for lipoprotein analysis as described in the next section. 

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