Analysis of Polysaccharides

Based on this background, a selected number of preparative, semipreparative, and analytical aqueous SEC systems for the analysis of polysaccharides, summarized in Table 16.1, are presented and discussed briefly in this chapter. 

Analysis of Polysaccharides by Ultracentrifugation. Size, Conformation and Interactions in Solution... 

Kvemheim, A.L. (1987). Methylation analysis of polysaccharides with butyllithium in dimethyl sulfoxide. Acta Chem. Scand. Ser. B41,150-152. 

Harding, S. E. Analysis of Polysaccharides by Ultracentrifugation. Size, Conformation and Interactions in Solution. Vol. 186, pp. 211-254. 

McCleary, B.V., and Matheson, N.K. (1986) Enzymic analysis of polysaccharide structure. Adv. Carbohydr. Chem. Biochem. 44, 147-276. 

The purification of a pneumococcal polysaccharide may be difficult, and the polysaccharide material is sometimes contaminated by a cell-wall component known as the C-substance. The methods for structural analysis of polysaccharides (which, like several of the pneumococcal polysaccharides, contain amino sugar and uronic acid... 

On the other hand, the method examines the rotations of contiguous residues about the glycosidic bonds, for example, C-l-O and O-C-4 in a (1 —> 4)-linked polysaccharide. This method scans the entire conformational space available to a polymer. The analysis can thus proceed either with a regular, helical structure, or a random conformation. The method has been used quite extensively in the conformational analysis of polysaccharides.9 26 The steric... 

Anhydrous hydrogen fluoride, application of for the structural analysis of polysaccharides, 47, 167-202 Anomeric and exo-anomeric effects in carbohydrate chemistry, 47, 45-123 Anomeric-oxygen activation for glycoside synthesis, trichloroacetimidate method, 50,21-123... 

How modeling has been useful in the crystal structure analysis of polysaccharides—and how it could lead to a better understanding of other condensed j)hase states—can be illustrated with structural worK done on cellulose. It is one of the world s most important and widely used raw materials whose structure, properties, derivatives, and transformations remain under continuous study. Some of the results, problems, and indications of future directions resulting from the study of its crystalline structure—and the attendant roles for molecular modeling—are briefly described in the following. 

---