Analysis of plasmids

Diogo, M.M., Queiroz, J.A., Monteiro, G.A., and Prazeres, D.M.F., Separation and Analysis of Plasmid Denatured Forms Using Hydrophobic Interaction Chromatography, Anal. Biochem., 275, 122, 1999. 

Schmidt, T., Friehs, K., Schleef, M., Voss, C., and Hasche, E., Quantitative analysis of plasmid forms by agarose and capillary gel electrophoresis, Anal. Biochem., 274, 235, 1999. 

Matsumoto Y, Itaka K, Yamasoba T, Kataoka K (2009) Intranuclear fluorescence resonance energy transfer analysis of plasmid DNA decondensation from nonviral gene carriers. J Gene Med 11 615-623... 

Can polyacrylamide gels be used for the analysis of plasmid DNA with greater than 3000 base pairs Why or why not ... 

Nagata, T., Uchijima, M., Yoshida, A., Kawashima, M. and Koide, Y. (1999) Codon optimization effect on translational efficiency of DNA vaccine in mammalian cells analysis of plasmid DNA encoding a CTL epitope derived from microorganisms. Biochem. Biophys. Res. Commun., 261, 445—451. 

Leamy VL, Martin T, Mahajan R, Vilalta A, Rusalov D, Hartikka J, Bozoukova V, Hall KD, Morrow J, Rolland AP, Kaslow DC, Lalor PA. Comparison of rabbit and mouse models for persistence analysis of plasmid-based vaccines. Hum Vaccines 2006 2 113-8. 

Trcek, J., Raspor, P., Teuber, M. 2000. Molecular identification of Acetobacter isolates from submerged vinegar production, sequence analysis of plasmid pJK2-l and application in the development of a cloning vector. Applied Microbiology and Biotechnology 53 289-295. 

Delivery of the plasmid to the target cells is only useful if the plasmid remains intact during the process and it is in a transcription-ready form after cellular internalization. As indicated earlier, it is not clear yet which plasmid conformation(s) can be defined as the active species. Any analysis of plasmids isolated from cells or tissues should take this into consideration bearing... 

All gel tanks, glassware, chemicals, and other equipment used should, if possible, be reserved for RNA preparations. Gel tanks that have been used for analysis of plasmid minipreps are often a source of RNases. Sterile disposable plasticware should be employed if possible as this is generally RNase-free. Gloves should be worn during preparation and purification procedures. These should be changed frequently. 

Whereas two-hybrid screening is utilized to identify interactions between two polypeptide molecules, reverse two-hybrid screening is to detect loss of the interactions. By this screening, it is possible to determine amino acid residues of given polypeptides that are crucial for particular protein-protein interactions. To isolate GAT domain mutants that lack interaction with Arf, we took advantage of reverse two-hybrid screening by a procedure as described above. Sequence analysis of plasmid clones isolated from colonies that developed pale blue color or did not develop blue color revealed that the insert cDNAs have frame shift mutations, single missense... 

Seo JH, Bailey JE. CeU cycle analysis of plasmid-containing Escherichia coli HBlOl populations with flow cytometry. Biotechnol Bioeng 1987 30 297—305. 

Middaugh, C.R., et al.. Analysis of Plasmid DNA from a Pharmaceutical Perspective. Journal of Pharmaceutical Sciences, 87 130-146,1998. 

Trcek J, Barja F (2015) Updates on quick identification of acetic acid bacteria with a focus on the 16S-23S rRNA gene internal transcribed spacer and the analysis of cell proteins by MALDI-TOF mass spectrometry. Int J Food Microbiol 196 137-144 Trcek J, Raspor P, Teuber M (2000) Molecular identification of Acetobacter isolates fiom submerged vinegar production, sequence analysis of plasmid pJK2-l and application in development of a cloning vector. Appl Microbiol Biotechnol 35 1899-1901 Trcek J, Toyama H, Czuba J, Misiewicz A, Matsushita K (2006) Correlation between acetic acid resistance and characteristics of PQQ-dependent ADH in acetic acid bacteria. Appl Microbiol Biotechnol 70(3) 366-373... 

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