Analysis of Dicumarol in Blood Serum

Stubbs et al. (1962) have devised a simple infrared method for the analysis of Dicumarol, 3,3 -methylene bis (4-hydroxycoumarin), in blood. The method is sensitive to concentrations as low as 0.1 mg % (1 ppm). The Dicumarol in acidified blood serum is extracted with carbon tetrachloride, and the solvent layer, containing the Dicumarol, is scanned in a 10-mm infrared microcell. The absorption at 1667 cm is measured and the concentration of the drug calculated. Compounds known to interfere with the determination of the drug in blood by other methods (e.g., vitamin K, salicylic acid, aspirin, warfarin, and others) do not interfere with the infrared method. 

American Petroleum Institute, Research Project 44 and the Manufacturing Chemists Association Research Project, located at Department of Chemistry of the Agricultural and Mechanical College of Texas. College Station, Texas. 

Chihara, G., Matsuo, K., Arimoto, K., Sugano, S., Shimizu, K., and Mashimo, K. Chem. Pharm. Bull. (Tokyo) 9, 939 (1961). 

Clarke, E. G. C. (ed.) Isolation and Identification of Drugs. The Pharmaceutical Press, London, 1969. Cook, D. and Regnier, Z. R. Can. J. Chem. 45, 2895 (1967a). 

Eglinton. G. in Physical Methods in Organic Chemistry (J. C. P. Schwarz, ed.) Holden-Day, Inc., San Francisco, 1964, p. 114. 

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