Analysis by Means of Radioisotopes

The following radiochemical analytical procedures can be distinguished according to the way in which the radioactive material is used [101, 387, 460, 611]  

These techniques have been often used in combination with PC there have been few instances to date of the use in TLC. 

This procedure is suitable for testing the resolution of chromatographic methods. 

One can demonstrate, for example, that the fractions of a naturally occurring lipid mixture, separated by TLC, are not contaminated by one another. This is shown in Fig. 94. A small amount of hot tripal-mitin was mixed with shark liver oil and separation then carried out with adsorption TLC. An autoradiograph of the chromatogram showed that the total radioactivity was in the triglyceride fraction. The glyceryl ether diesters were not contaminated with the triglycerides despite their very similar structure. 

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It may be pointed out here that non-radioactive compounds can be detected autoradiographically after neutron activation or in the form of labelled derivatives. These procedures are discussed in more detail in section V, Analysis by Means of Radioisotopes . 

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