Analysis by Fluorescent Indicator in Liquid Chromatography

This analysis, abbreviated as FIA for Fluorescent Indicator Adsorption, is standardized as ASTM D 1319 and AFNOR M 07-024. It is limited to fractions whose final boiling points are lower than 315°C, i.e., applicable to gasolines and kerosenes. We mention it here because it is still the generally accepted method for the determination of olefins. 

Chapter 3. CHARACTERIZATION OF CrUDB OiLS AND PETROLEUM FRACTIONS 

As stated earlier, these hydrocarbons are difficult to quantify with accuracy. The FIA method, which is a chromatographic adsorption on silica, gives volume percentages of saturated hydrocarbons, olefins and aromatics. 

Some attempts, not yet very convincing, have been made to extend the field of application to gas oils by heating the column. 

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