Analgesics column chromatography

The recent development of commercial HPLC systems has provided a powerful instrumentation for the separation, characterization, identification, and quantitation of minute amounts of essential dietary components (68,69). Developments in hardware and packings for HPLC have overcome the problems of nonreproducible behavior and low efficiency separations previously associated with column chromatography (70). HPLC has already been applied to the quantitative analysis of analgesics, pesticides, and fat-soluble vitamins with precision and accuracy and a minimum of sample clean-up. Such instrumentation provides a rapid, accurate, and sensitive technique for the separation and analysis of subnanomole quantities of a wide range of complex high-molecular-weight, nonvolatile, thermally labile, compounds that are vital for metabolic and nutritional studies. [Pg.205]

Experiment 14. Purification of proteins on DEAE-cellulose Experiment 15. Separation of a mixture of analgesics by flash column chromatography... [Pg.1]

Separation of a mixture of analgesics by flash column chromatography. [Pg.428]

Flash column chromatography, normal phase, analgesics. [Pg.456]

Oxyphenbutazone, and other non-narcotic analgesics are screened by reverse-phase high-pressure liquid chromatography (39). The column is LiChrosorb RP8 and the mobile phase is a acetonitrile-water mixture. Blood and organs are homogenized in 4% perchloric acid before extraction with the solvents. Urine is extracted without primary treatment. [Pg.357]

P. Cockaerts, E. Roets, and J. Hoogmartens, Analysis of a complex analgesic formulation by high performance liquid chromatography with column-switching, J. Pharm. Biomed. Anal., 4 361 (1986). [Pg.108]

To 0.5 ml of urine in a micro test-tube add 100 Lil of 2M sodium hydroxide, and 100 li1 of chloroform, mix for 30 seconds, centrifuge in a high-speed centrifuge for 2 minutes, and inject 3 to 5 Lil of the chloroform extract on to both columns. Identify any peaks which appear by reference to the retention data listed in Table 17. Furdier systems and data for the gas Chromatography of narcotic analgesics are given on p. 198. [Pg.30]

Di Pietra, A.M. Gatti, R. Andrisano, V. Cavrini, V. Application of high-performance liquid chromatography with diode-array detection eind on-hne post-column photochemical derivatization to the determination of analgesics. J.ChromatognA, 1996, 729, 355-361... [Pg.11]

Saarinen, M.T. Siren, H. Riekkola, M.-L. Screening and determination of p-blockers, narcotic analgesics and stimulants in urine by high-performance liquid chromatography with column switching. J.Chromatogr.B, 1995, 664, 341-346... [Pg.161]

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