An Introduction to Solution, Solid-State, and Imaging NMR Spectroscopy

Nuclear magnetic resonance is a flexible technique with many applications [1, 2]. For substances dissolved in solution, NMR observation of H, N, and 

In synthetic organic and organometaUic chemistry, solution-state NMR means a 300—500 MHz NMR spectrometer, high-precision glass sample tubes, 2 ml of deuterated solvent (typicaUy fully deuterated chloroform, acetone, benzene, or dichlorobenzene), several milligrams of pure sample, and a basic suite of H and NMR experiments [3 7]. With several hours of spectrometer time and data interpretation, the stuctures of new compounds with molecular weights up to 2000 Da can be determined, espedaUy when analyzed along with results from NMR databases and mass spectroscopy. 

The structure of a protein in solution usuaUy compares well with a crystallographic determination. However, not all proteins crystallize, and crystals of membrane-bound proteins are especiaUy rare. Hence, the 600 800 MHz and newly constructed 900 MHz NMR spectrometers are largely aUocated for biological samples [8, 9]. Although most proteins are studied in solution, membrane-bound proteins may be studied in assembled bilayers. Currently, the greater part of NMR technol- 

Han Mok of Spectroscopy Volume 1. Edited by Gunter Gauglilz and Tuan Vo-Dinh Copyright 2003 WILEY-VCH Verlag GmbH Co. KGaA, Weinheim ISBN 3-527-297S2-0 

40 MHz 1 m open-access permanent magnet whole body imaging 

---

In the opening chapter, An Introduction to Solution, Solid-State, and Imaging NMR Spectroscopy Leslie Butler (Louisiana State University) introduces the fundamental structure parameters in the NMR experiment through a discussion on solution state H NMR. The shielding of nuclei by core and valence electrons, gives rise to those structure-pregnant numbers called chemical shifts, d, that have been accrued and correlated since the earliest days of NMR. Scalar coupling,... 

An Introduction to Solution, Solid-State, and Imaging NMR Spectroscopy... 

An Introduction to Solution, Solid-State, and Imaging NMR Spectroscopy 183 Table 7.2 Calculated H NMR transitions for 1-chloroethene at 6 = 2.3488 I... 

---