Amylose sulfate preparation

High temperatures can break native S-S bonds and form new S-S bonds which can lock the protein into a denatured eonfiguration [89]. Low pH, sodium dodecyl sulfate. Tween 80, chaotropie salts, and exogenous proteins have been used to protect proteins from thermal inaetivation [90]. Ethylene glycol at 30-50% was used to protect the antiviral activity of P-interferon preparations [91]. Human serum albumin was used in recombinant human interferon-Psei-n which resulted in increased thermal stability [62]. Water-soluble polysaeeharides sueh as dextrans and amylose [92], as well as point-specific (site-directed) mutagenesis [93] have also been used to increase thermal stability of therapeutie proteins and peptides. 

The location of the metal cation (or cations) in alcoholates has been investigated by the method of substitutive methylation for the sodium derivatives of methyl a-D-glucopyranoside, amylose, and cellulose. The method assumes that methylation by either dimethyl sulfate or methyl iodide occurs only at an anionic oxygen atom. Lenziu methylated the mono-sodium alcoholates (prepared in boiling, butanolic sodium hydroxide by the... 

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