Amplification refractory mutation detection system

The most widely used genetic screening technique, PCR-RFLP detects a mutation at a specific restriction endonuclease cleavage site at the mutation locus [9], The products from other techniques such as ARMS (amplification refractory mutation system), SSCP (single-strand conformational polymorphism), HPA (heteroduplex polymorphism), and CDCE (constant denaturant capillary electrophoresis) and PCR are usually separable using polymer networks. 

To analyze DNA mutations directly in the CF gene a technique can be used which is widely applicable in many areas of DNA analysis and mutation detection. This is the amplification refractory mutation system (ARMS). 

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