Amplification refractory mutation detection

The most widely used genetic screening technique, PCR-RFLP detects a mutation at a specific restriction endonuclease cleavage site at the mutation locus [9], The products from other techniques such as ARMS (amplification refractory mutation system), SSCP (single-strand conformational polymorphism), HPA (heteroduplex polymorphism), and CDCE (constant denaturant capillary electrophoresis) and PCR are usually separable using polymer networks. 

To analyze DNA mutations directly in the CF gene a technique can be used which is widely applicable in many areas of DNA analysis and mutation detection. This is the amplification refractory mutation system (ARMS). 

Selective PCRs for the detection of HIV-1 drug resistance mutations have first been described by Larder et al. (3), who called it amplification refractory... 

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