Ampicillin fluorometric detection

Direct nonchromatographic methods target screening of either ampicillin residues in milk extracts by fluorometric detection (126) or -lactam residues in kidney extracts by photometric detection (120). These methods are rapid but cannot differentiate among particular -lactams and are less sensitive than other screening tests usually employed for regulatory purposes. 

Fluorometric detection has been mainly employed for the determination of aminopenicillins such as amoxicillin and ampicillin in edible animal products because it confers the advantages of selectivity and sensitivity. Fluorometric detection of penicillins, however, necessitates their precolumn derivatization to produce the corresponding fluorescent derivatives. The most commonly used derivatizing reagents are formaldehyde (100, 117, 118), salicylaldehyde (83), and mercury dichloride (91). 4-Bromomethyl-7-methoxycoumarin has also been employed as a fluorescence label for the selective and sensitive detection of seven penicillins in milk (96). 

Miyazaki, K, Ohtani, K. Sunada, K. Arita, T. Determination of ampicillin, amoxicillin, cephalexin, and cephradine in plasma by high-performance liquid chromatography using fluorometric detection. J.Chromatogr., 1983, 276, 478—482... 

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See also in sourсe #XX -- [ ]

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