2-Aminonaphthalene-4,6,8-trisulfonic acid

Larsson, M., Sundberg, R. and Folestad, S. On-line capillary electrophoresis with mass spectrometry detection for the analysis of carbohydrates after derivatization with 8-aminonaphthalene-1,3,6-trisulfonic acid. / Chromatogr A, 934, 75, 2001. 

Precolumn fluorescent derivatization 5-Aminonaphthalene-1-sulfonic acid (ANSA) 7- Aminonaphthalene-1,3-disulfonic acid (ANDSA) 8- Aminonaphthalene-1,3,6-trisulfonic acid (ANTS) Fluorescein isothiocyanate (FITC) 9- fluroenylmethyl chloroformate All the analytes ANSA enhances the enantio-separation... 

Chiesa, C. and Horvath, Cs., Capillary zone electrophoresis of malto-oligosac-charides derivatized with 8-aminonaphthalene-l,3,6-trisulfonic acid,. Chromatogr., 645, 337, 1993. 

Quenched fluorophores are encapsulated in liposomes and exposed to the inhibitors in the desired concentrations. If leakage of liposomes occurs, fluorescence intensity should increase significantly. Examples for applied markers are (i) self-quenching calcein (<60mM) (ii) HPTS (35mM) with the quencher DPX (50mM) (126,128) and (hi) 8-aminonaphthalene-l,3, 6-trisulfonic acid (ANTS) and DPX. 

The acid solution is neutralized by adding calcium carbonate, and the precipitated gypsum produced from the excess sulfuric acid is filtered off. The nitrated product remains in the filtrate and is reduced by iron chippings to T acid (1-aminonaphthalene-3,6,8-trisulfonic acid). The remaining calcium ions are removed as calcium carbonate together with the iron oxide by adding sodium carbonate solution, and the T acid is isolated as an intermediate by acidification of the filtrate, so that the various byproducts can be removed with the mother liquor. 

Kits for analyzing glycoproteins are now available, utilizing recombinant endo-enzymes together with electrophoresis and fluorescent labeling with ANTS (aminonaphthalene-l,3,6-trisulfonic acid) PMP (1-phenyl-3-methyl-5-pyrazolone), or other, similar type of reagent can be used. MS plays a crucial role in glycopeptide characterization where techniques such as electrospray LC-MS and MALDI-TOF MS are utilized. 

J-acid can be obtained from Tobias acid by sulfonation with 30% oleum at 100 °C to 2-aminonaphthalene-l,5,7-trisulfonic acid, hydrolytic removal of the sulfonic acid group in the 1-position, and alkali fusion. 

Zou et al. [1560] separated 14 phenylamine- and naphthylaminesulfonic acids (e.g., 2-aminonaphthalene-4,6,8-trisulfonic acid, l,3 diamino-4-sulfonic acid, phenylamine-2-sulfonic acid, 6-chlorophenylamine-3-sulfonic acid, naphthylamine-7-sulfonic acid) in 30 min on a Cjg column (A = 254 nm). Baseline resolution was achieved using an aqueous 10 mM phsophate buffer pH 6.8 mobile phase. The capacity ctors for these and 10 additional compounds belonging to these classes of compounds were tabulated. 

Five impurities of 8-amino-l-naphthol-3,6-disulfonic acid (e.g., l-naphthol-4-sulfonic acid, 8-aminonaphthalene-l,3,6-trisulfonic acid, chromotropic acid) were resolved on a C,g column (A = 235 nm) using an aqueous 0.3 M sodium sulfate mobile phase [1561]. All compounds eluted in <8 min. Detection limits of... 

In addition to its importance in the mechanism of formation of naph-thalene-2-sulfonates, the desulfonation reaction has preparative utility in producing the dye intermediate 6-aminonaphthalene-l,3-disulfonic acid from the 1,3,5-trisulfonate. This is accomplished by refluxing at 125°C for 4 hr in dilute sulfuric acid. 

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